HPLC This article provides a comprehensive exploration of the intricate mechanism behind the separation of components using High-Performance Liquid Chromatography (HPLC). HPLC is a powerful analytical technique widely used in various industries, particularly in pharmaceuticals and chemistry. The article delves into the principles governing the separation process, covering factors such as stationary phases, mobile phases, and detector interactions. Whether you're a researcher, analyst, or enthusiast, this guide offers valuable insights into the inner workings of HPLC, empowering readers to make informed decisions in analytical science.